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静磁场增强3D打印多孔钛支架上BMSc成骨分化的研究

 

 

10.1096/fj.201802195R

 

大型骨缺损的重建是骨科医师面临的重大挑战。三维打印支架被认为是一种很有前途的修复材料。静磁场(SMF)是一种有效且无创的治疗方法,已被证明可以加速骨折愈合,促进骨再生。然而,SMF对人骨髓间充质干细胞(hBMSCs)3DP支架中的成骨作用及其潜在机制尚不清楚。

在本研究中,邱贵兴教授带领其团队研究了体内外SMFhBMSCs3DP支架内的成骨作用。此外,通过蛋白组学分析探讨了SMF促进成骨的潜在机制。实验结果表明SMF促进hBMSCs体外成骨分化。通过蛋白组学分析,在SMF条件下共鉴定出185种差异蛋白。成骨作用可能与SMF条件下激活骨形态发生蛋白-Smad1/5/8信号通路和通过上调Smad4增加转运磷酸化Smad1/5/8和磷酸化Smad2/3至细胞核有关。体内实验表明,SMF能促进骨缺损大鼠的骨再生和骨整合。总之,中度SMF是一种安全有效的促进3DP支架体内外成骨的方法。

Enhanced osteogenic differentiation of human bone–derived mesenchymal stem cells in 3-dimensional printed porous titanium scaffolds by static magnetic field through up-regulating Smad4. The reconstruction of large bone defects remains a significant challenge for orthopedists. Three dimensional– printed (3DP) scaffold is considered a promising repair material. Static magnetic field (SMF) is an effective and noninvasive therapy method that has been shown to speed up bone fracture healing, stimulate bone regeneration. However, the osteogenic effect of SMF on human bone–derived mesenchymal stem cells (hBMSCs) in 3DP scaffolds, as well as its potential mechanism, are unclear.

In this study, the osteogenic effect of SMF on hBMSCs in a 3DP scaffold was investigated in vitro and in vivo by Guixing Qiu professor and his team. In addition, the potential mechanism for promoting osteogenesis was investigated by proteomic analysis. The results showed that SMF promoted osteogenic differentiation of hBMSCs in vitro. A total of 185 differential proteins were identified under SMF conditions by proteomic analysis. The osteogenic effect might be associated with bone morphogenetic protein-Smad1/5/8–signaling pathway and increased transport of phosphorylated Smad1/5/8 and phosphorylated Smad2/3 to the nucleus by up-regulating Smad4 under SMF conditions. The in vivo experiment showed that bone regeneration and osseointegration was enhanced by SMF in the rat model of bone defect. In conclusion, moderate SMF was a safe and effective method for enhancing osteogenesis in 3DP scaffolds in vitro and in vivo.

郝莉莉

 

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