Biomaterials 188 (2019) 198-212
干细胞球状体在器官培养和治疗移植中得到了广泛的研究。来自韩国首尔的Se-jeong Kim等通过设计一种表面压纹结构的水凝胶，构建可以快速形成和大量培养干细胞球的一体化平台。水凝胶上的浮雕结构可根据砂纸的粒度指定进行调整。人类脂肪干细胞(hADSCs)水凝胶快速组装成球状体,根据凝胶表面粗糙度，他们的大小范围控制在从95±6μm 到181±15μm。此种方法制备的干细胞球维持了较好的多向分化潜能，同时，与普通商业低接触培养板培养相比，此细胞球VEGF的表达明显提高。利用这些优点，作者将基于HES的球状体用于3D打印，3D打印结构中的球状体与普通的单细胞3D打印结构相比，具有更好的干性保留和VEGF分泌能力。总的来说，HES将为干细胞球状体的大规模制造和培养提供一个有用的平台，这些球状体的尺寸可控，可用于各种生物医学应用。
Stem cell spheroids have been studied extensively in organoid culture and therapeutic transplantation.Se-jeong Kim from Seoul designed an all-in-one platform that can enable the rapid formation and culture of a large quantity of size-controllable stem cell spheroids according to the hydrogels with an embossed surface (HES) . The embossed structure on the hydrogel was adjustable according to the grit designation of the sandpaper. Human adipose-derived stem cells (hADSCs) were rapidly assembled into spheroids on the hydrogel, with their size distribution precisely controlled from 95 ± 6 μm to 181 ± 15 μm depending on surface roughness.The hADSC spheroids prepared from the HES demonstrated expression of stemness markers and differentiation capacity. In addition, HES-based spheroids showed significantly greater VEGF secretion than spheroids grown on a commercially available low-attachment culture plate.Exploiting those advantages, the HES-based spheroids were used for 3D bioprinting, and the spheroids within the 3D-printed construct showed improved retention and VEGF secretion compared to the same 3D structure containing single cell suspension.Collectively, HES would offer a useful platform for mass fabrication and culture of stem cell spheroids with controlled sizes for a variety of biomedical applications.