Recently, the repair and treatment of large bone defect has been widely discussed in clinic research. There were three main types of graft options, including autogenous bone, allogeneic bone, and synthetic materials.They all had their pros and cons. The CaPcs could simulate the strength of the autologous bone and was proved to have good biocompatibility, osteoconductivity and osteoinductivity. In particular, its osteoinductivity has been widely concerned. Vitro cell experiments have proved the idea of signaling pathways and protein expression, however, there is still a lack of authoritative vivo experiments.
Recently, the author's team designed a complete vivo test based on its vitro test results. First, four kinds of calcium phosphate materials were synthesized and characterized. They then modeled the mice for vivo experiments. Histological and microscopic changes were observed at 4 and 16 weeks. Finally, they quantitatively analyzed several osteogenic mRNA and proteins screened by PCR and IHC. They concluded that CaPcs could be proved to have osteoinductivity from gene, protein and tissue levels. In addition, the strength and weakness of bone induction were closely related to the phase composition.