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多孔壳聚糖微载体三维细胞培养应用

 

 

 

 

 

Carbohydrate Polymers 202 (2018) 611–620
 

 

   据报道,许多微载体只能在其外表面实现细胞生长,一些多孔类微载体因使用有毒交联剂和化学致孔剂,存在细胞毒性、空隙不够大,不足以容纳细胞生长等问题
        最近,Lixia Huang等采用乳化热诱导相分离法(TIPS)制备了高孔隙壳聚糖微球(CSM),制备过程中未使用除冰以外的有毒交联剂和化学致孔剂。其平均直径为150μm,连通孔大小20~50μm之间。由于其良好的生物相容性和独特的多孔结构,使用CSM作为微载体实现了肝细胞的三维高效培养,细胞不仅生长于微载体的外表面,同时良好的生长于内部孔径中,并观察到多向细胞间的相互作用。与2D细胞培养相比,CSM微载体增强了细胞的活性和功能。相信这种微载体会为体外三维细胞培养提供一个很有前景的平台。

Many microcarriers were reported that only achieved cell growth on their outer surface. Some porous microcarriers have some problems such as cytotoxicity due to the use of toxic crosslinking agents and chemical pore-forming agents. The gap is not large enough to accommodate cell growth and so on.

Herein, Lixia Huang et al used emulsion-based thermally induced phase separation (TIPS) to prepare highly porous chitosan microspheres (CSM) without using toxic crosslinkers and chemical porogenic agents other than ice. The CSM had an average diameter of 150μm with interconnected pores varying from 2050μm in size. Due to their excellent biocompatibility and unique porous structure, high-performance hepatocyte culture in three-dimensional (3D) space was achieved using the CSM as microcarriers, as cell growth also took place within the internal pores of the CSM, besides their external surface, and multidirectional cell–cell interactions were observed. Enhanced cellular activity and functions were obtained with the CSM microcarriers as compared with 2D cell culture. It is believed that these CSM microcarriers provide a promising platform for 3D cell culture in vitro.

 

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