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玉米醇溶蛋白微载体的制备及其在细胞培养中的应用

 

 

 

 

 

Materials Science and Engineering C 58 (2016) 863–869

 

 

 

在大规模细胞培养中,具有较大比表面积的微载体受到了广泛的关注。微载体的制备主要采用乳化法、悬浮聚合法、溶剂蒸发法、有机相分离法(内聚法)和喷雾干燥法。其中许多需要使用有机溶剂。一般来说,有机溶剂被认为是有毒的,不利于环境保护。

 

上海交通大学生物医学工程学院王教授团队以甘油为分散介质,采用一种新的方法成功制备了玉米醇溶蛋白微载体。这些微载体的尺寸是可控的,并且制备过程执行起来很简单。Vero细胞作为疫苗生产中常用的细胞,在玉米醇溶蛋白微载体表面进行培养,以评价玉米醇溶蛋白微载体在细胞培养中的作用。细胞在玉米醇溶蛋白微载体表面的粘附和扩增,与Cytodex 1表面的细胞无明显差异。细胞密度在第4天达到最大值,约为初始密度的10倍。这是第一篇关于玉米醇溶蛋白可作为大规模悬浮细胞培养的微载体的报道,表明了玉米醇溶蛋白替代商用不可降解材料的潜力。

 

Microcarriers with a large surface area have attracted much attention in the field of large-scale cell culture. The preparation of microcarriers is mainly based on methods such as emulsification, suspension polymerization, solvent evaporation, organic phase separation (cohesion) and spray drying. Many of these require the use of organic solvents. In general, organic solvents are considered to be toxic and not conducive to environmental protection.

 

Zein microcarriers were successfully prepared by the team of Professor Wang Jinye, School of Biomedical Engineering, Shanghai Jiao Tong University, using glycerol as dispersing medium. The size of these microcarriers was controllable, and the preparation process was simple to perform. Vero cells, commonly used in the production of vaccines, were cultured on the surface of the zein microcarriers to evaluate the microcarriers' performance in cell culture. The cells could attach and spread on the surface, without any difference compared with cells on the surface of Cytodex 1. The cell density reached its maximum on the fourth day, which was approximately 10 times the initial density. This is the first report that zein can be used as a microcarrier for the mass production of a large-scale suspension cell culture, which shows the potential of zein as a replacement for commercial non-degradable materials.

 

 

(丛政)

 

 

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