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Zn²多孔壳聚糖微球用来增强血栓的形成和止血

 

Materials Science & Engineering C 85 (2018) 27–36

 

 

在战场和平民事故中,非致命性大规模创伤性出血的快速止血方法,对于降低死亡率和减少医疗费用非常重要。壳聚糖(CS)作为临床止血剂被广泛的应用,为了提高其止血效率,Zn2以海藻酸锌(ZnAlg)的形式引入CS,使其表面具有ZnAlg成分的多孔CS@ZnAlg微球。该微球是由微乳液、聚电解质附着力和热诱导相分离等连续步骤制备的。CS@ZnAlg微球具有内外相互联通的多孔结构,他们的大小、表面积和吸水率都比单纯的壳聚糖微球要好。CS@ZnAlg微球的血栓形成时间较短,体内和体外全血凝血的时间更快,血凝块更大,失血更少。CS@ZnAlg微球止血原理为(1)壳聚糖组分与红细胞之间的静电吸引作用(2Zn²藻酸盐组分激活凝血因子XⅡ3)微球基质的物理阻断作用,促进了CS@ZnAlg微球的止血性能。

最近福建师范大学王正超团队成功合成了CS@ZnAlg微球。通过SEM, FT-IR, XPS等表征验证了该材料的合成,以及通过鼠肝脏裂伤,尾部断裂实验很好的验证了该CS@ZnAlg微球良好的止血效果。

Quick hemostats for non-lethal massive traumatic bleeding in battleeld and civilian accidents are important for reducing mortality and medical costs. Chitosan (CS) has been widely used as a clinic hemostat. To enhance its hemostatic efficiency, Zn²⁺ in the form of zinc alginate (ZnAlg) was introduced to CS to make porous CS@ZnAlg microspheres. The composite microspheres had an outer and internal interconnected porous structure. Their size, surface area, and water absorption ratio Compared to the neat chitosan microspheres, the CS@ZnAlg microspheres showed shorter onset of clot formation, much faster in vitro and in vivo whole blood clotting, bigger clot, less blood loss, and shorter hemostatic time in the rat liver laceration and tail amputation models. The synergetic hemostatic effects from (1) the electrostatic attraction between chitosan component and red blood cells, (2) the activation of coagulation factor XII by Zn²⁺ of zinc alginate component, and (3) physical blocking by microsphere matrix, contributed to the enhanced hemostatic performance of CS@ZnAlg microspheres.

Recently, Fujian Provincial Key Laboratory of Polymer Materials, Fujian Normal University Zhengchao Wang team successfully synthesized CS@ZnAlg microsphere the synthesized of this material was verified by SEMFTIRXPS and other characterization, as well as the rat liver lacerated and tail fracture experiment was good to verify the hemostatic effect of the CS@ZnAlg microsphere.

(吕彩莉)

 

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