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压电性聚(3-羟基丁酸-co-3-羟基戊酸共聚酯)的制备及在骨组织工程中的应用

 

 

 

 

Colloids & Surfaces B Biointerfaces, 2017, 160:48-59.

 

在以往的骨修复材料研究中,人们往往关注材料的生物降解性,生物相容性,力学性能,多孔结构和表面性质,但对材料的压电性在骨修复中的应用研究较少。

 

最近,托木斯克理工大学Svetlana N. Gorodzha团队采用静电纺丝的方法合成了PCL,压电材料PBHV,压电材料PBHV-SiHA三种纤维支架。并研究了材料的表面形貌,化学组成,润湿性和生物学性能。将SiHA加入PBHV材料中可以增加纤维的尺寸和材料表面的粗糙程度。为测试材料压电性和掺杂SiHA后对细胞黏附和增殖的影响,将人骨髓间充质干细胞在三种材料上进行培养。体外研究细胞的活性,增殖能力,成骨分化情况,结构显示PBHV-SiHA材料表面的细胞具有很好的黏附和增殖情况。此外具有压电性的PBHV比无压电性的PCL材料更促进细胞的矿化。研究结果显示混合PHBV-SiHA支架用于骨组织工程的有着显著优点。

In the past studies of bone repair materials, people tend to focus on biodegradability, biocompatibility, mechanical properties, porous structure and surface properties of materials, but less research on the application of piezoelectricity in bone engineering.

In this study, bone scaffolds composed of polycaprolactone (PCL), piezoelectric poly(3-hydroxybutyrateco-3-hydroxyvalerate) (PHBV) and a combination of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) and silicate containing hydroxyapatite (PHBV-SiHA) were successfully fabricated by a conventional electrospinning process. The morphological, chemical, wetting and biological properties of the scaffolds were examined. Fibre size increased with the addition of SiHA to PHBV scaffolds. Moreover, fibre surface roughness in the case of hybrid scaffolds was also increased. To determine the influence of the piezoelectric nature of PHBV in combination with SiHA nanoparticles on cell attachment and proliferation, PCL (nonpiezoelectric), pure PHBV, and PHBV-SiHA scaffolds were seeded with human mesenchymal stem cells (hMSCs). In vitro study on hMSC adhesion, viability, spreading and osteogenic differentiation showed that the PHBV-SiHA scaffolds had the largest adhesion and differentiation abilities compared with other scaffolds. Moreover, the piezoelectric PHBV scaffolds have demonstrated better calcium deposition potential compared with non-piezoelectric PCL. The results of the study revealed pronounced advantages of hybrid PHBV-SiHA scaffolds to be used in bone tissue engineering.

 

(王鹏)

 

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