In recent years the potential application of nanocomposite biomaterials in tissue engineering field is gaining importance because of the combined features of all the individual components.Indian Institute of Technology Kharagpur made a bottom-up approach is acquired in this study to recreate the bone microenvironment .The major rationale behind bone tissue engineering is the need for reconstruction of biodegradable bioactive non-immunogenic orthopedic implants with better filler material and mechanical strength close to the biological anatomy.
Non-mulberry tropical tasar Antheraea mylitta is considered as an important natural polymer amongst several synthetic polymers for supporting as an extracellular matrix (ECM) for bone. The inherent integrin binding tripeptide -Arg-Gly-Asp- motif [UniProtKB _ Q81SB3: fiveRGD motifs present in the partial CDS of 507 amino acids] aids firm cellular adhesion. Carbon nanofiber are already known for their pronounced electrical, mechanical,thermal, and structural properties. dual growth factor (BMP-2 and TGF-b1) loaded three dimensional CNF reinforced nonmulberry silk protein fibroin nanocomposite sponges and investigating their potential as bone tissue construct for the first time. The study clearly shows the potential attributes of these composite matrices as an extra cellular matrix for supporting successful osseointegration process.