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RSC Adv.,2016,6,20202-20210 DOI:  10.1039/C5RA27914C

Photo-immobilization of bone morphogenic protein 2 on PLGA/HA nanocomposites to enhance the osteogenesis of adipose-derived stem cells

 Tianlin Gao,ab Weiwei Cui,b Zongliang Wang,*a Yu Wang,a    Ya Liu,*b  Ponnurengam Sivakumar Malliappan,cd Yoshihiro Itocd and Peibiao Zhang*a  
 
Author information
* Corresponding authors
 a  Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, China
 E-mail:  zhangpb@ciac.ac.cn, wangzl@ciac.ac.cn
 b  School of Public Health, Jilin University, Changchun, P. R. China
 E-mail:  liuya@jlu.edu.cn
 c  Nano Medical Engineering Laboratory, RIKEN, 2-1-Hirosawa, Wako, Saitama 351-0198, Japan
 d  Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Materials Science, 2-1-Hirosawa, Wako, Saitama 351-0198, Japan
 
Abstract
Lack of bioactivity has seriously restricted the development of biodegradable implants in bone tissue engineering. In this study, Bone Morphogenic Protein 2 (BMP-2) was photo-immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA composites via photo-reactive gelatin (Az-Gel). The results demonstrate that the Az-Gel facilitates efficient and long-term immobilization of peptides on the surface of PLGA. The immobilization of Az-Gel enhanced the adhesion and proliferation of Mouse Adipose-Derived Stem Cells (ADSCs), while HA incorporation facilitated cellular osteodifferentiation. The immobilization of BMP-2 induced the osteogenesis of the ADSCs, indicated by alkaline phosphatase activity, quantitative real-time polymerase chain reaction (qRT-PCR) analysis and mineralization on the deposited substrates. Furthermore, the surface immobilization of BMP-2 could not only reduce the amount of growth factor, but also have a long-term osteoinductive effect. Therefore, it is considered that the surface modification method has a great potential for the enhancement of osteointegration of biodegradable bone implants.
 
Graphical abstract: Photo-immobilization of bone morphogenic protein 2 on PLGA/HA nanocomposites to enhance the osteogenesis of adipose-derived stem cells
 
KEYWORDS:
adhesion; collagen mimetic peptide; composite; dopamine; osteogenesis; osteogenic growth peptide
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