Mater Sci Eng C Mater Biol Appl. 2018 Sep 1;90:168-179. doi: 10.1016/j.msec.2018.04.061. Epub 2018 Apr 22.
Intracellular calcium ions and morphological changes of cardiac myoblasts response to an intelligent biodegradable conducting copolymer.
Wang Y 1, Zhang W 2, Huang L 1, Ito Y 3, Wang Z 1, Shi X 1, Wei Y 4, Jing X 1, Zhang P 5.
1 Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China.
2 School of Life Sciences, Northeast Normal University, Changchun 130022, China.
3 Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako-shi Saitama, 351-0198, Japan.
4 Department of Chemistry, Tsinghua University, Beijing 100084, China.
5 Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China. Electronic address: firstname.lastname@example.org.
A novel biodegradable conducting polymer, PLA-b-AP-b-PLA (PAP) triblock copolymer of poly (l-lactide) (PLA) and aniline pentamer (AP) with electroactivity and biodegradability, was synthesized and its potential application in cardiac tissue engineering was studied. The PAP copolymer presented better biocompatibility compared to PANi and PLA because of promoted cell adhesion and spreading of rat cardiac myoblasts (H9c2 cell line) on PAP/PLA thin film. After pulse electrical stimulation (5 V, 1 Hz, 500 ms) for 6 days, the proliferation ratio, and intracellular calcium concentration of H9c2 cells on PAP/PLA were improved significantly. Meanwhile, cell morphology changed by varying the pulse electrical signals. Especially, the oriented pseudopodia-like structure was observed from H9c2 cells on PAP/PLA after electrical stimulation. It is regarded that the novel conducting copolymer could enhance electronic signals transferring between cells because of its special electrochemical properties, which may result in the differentiation of cardiac myoblasts.
Copyright © 2018. Published by Elsevier B.V.
Aniline pentamer; Biocompatibility; Cardiac tissue engineering; Electrical stimulation; H9c2 cell; Poly(l-lactide)
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