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Macromol Biosci. 2015 Aug;15(8):1070-80. doi: 10.1002/mabi.201500069. Epub 2015 May 7.

Biodegradable Microcarriers of Poly(Lactide-co-Glycolide) and Nano-Hydroxyapatite Decorated with IGF-1 via Polydopamine Coating for Enhancing Cell Proliferation and Osteogenic Differentiation.

Gao T 1,2, Zhang N 3, Wang Z 2, Wang Y 2, Liu Y 4, Ito Y 5,6, Zhang P 7.

Author information

1School of Public Health, Jilin University, Changchun, 130021, P. R. China.
2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China.
3Department of Foot and Ankle Surgery, The Second Hospital of Shandong University, Jinan, 250000, P. R. China.
4School of Public Health, Jilin University, Changchun, 130021, P. R. China. liuya@jlu.edu.cn.
5Nano Medical Engineering Laboratory, RIKEN, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.
6Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Materials Science, 2-1-Hirosawa, Wako, Saitama, 351-0198, Japan.
7Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, P. R. China. zhangpb@ciac.ac.cn.


Abstract

In this study, insulin-like growth factor 1 (IGF-1) was successfully immobilized on the poly(lactide-co-glycolide)/hydroxyapatite (PLGA/HA) and pure PLGA microcarriers via polydopamine (pDA). The results demonstrated that the pDA layer facilitated simple and highly efficient immobilization of peptides on the microcarriers within 20 min. Mouse adipose-derived stem cells (ADSCs) attachment and proliferation on IGF-1-immobilized microcarriers were much higher than non-immobilized ones. More importantly, the IGF-1-immobilized PLGA/HA microcarriers significantly increased alkaline phosphatase (ALP) activity and expression of osteogenesis-related genes of ADSCs. Therefore, it is considered that the IGF-1-decorated PLGA/HA microcarriers will be of great value in the bone tissue engineering.


 

KEYWORDS:

IGF-1; biodegradable PLGA microcarriers; bone tissue engineering; nano-hydroxyapatite; polydopamine

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