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Carbohydr Polym. 2017 Oct 15;174:723-730. doi: 10.1016/j.carbpol.2017.05.087. Epub 2017 Jun 4.

A chitin film containing basic fibroblast growth factor with a chitin-binding domain as wound dressings.
 
Wang Y1, Fu C2, Wu Z3, Chen L3, Chen X4, Wei Y5, Zhang P6.
 
Author information
1Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China.2Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China; Department of Orthvopaedic Surgery, The Second Hospital of Jilin University, Changchun 130041, People's Republic of China.3Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China; Department of Pharmacy, Jilin University, Changchun 130012, People's Republic of China.4Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.5Department of Chemistry, Tsinghua University, Beijing 100084, People's Republic of China.6Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China. Electronic address: zhangpb@ciac.ac.cn.

Abstract
Basic fibroblast growth factor (bFGF) can stimulate wound healing. However, consistent delivery of bFGF has many disadvantages. To decrease their instability and diffusible, we introduced chitin-binding domain (ChtBD) into bFGF. Two expression plasimids were constructed. The first one (named bFGF) contained bFGF (154 amino acids), the second (named ChtBD-bFGF) contained bFGF and the ChtBD (54 amino acids). ChtBD was derived from chitinase A1 (ChiA1) of Bacillus circulans WL-12. The recombinant protein ChtBD-bFGF had the same biological activity as bFGF in promoting fibroblast proliferation. Chitin powder was dissolved in 11wt% NaOH and 4wt% urea aqueous solution via the freezing/thawing method. A chitin solution was spread on a glass plate and coagulated with anhydrous alcohol. The chitin binding ability of ChtBD-bFGF was 11.4-fold higher (up to 286μg/cm2) than bFGF in vitro. The immunofluorescence data indicated that the ChtBD-bFGF@chitin film promoted cell adhesion and proliferation. The ChtBD-bFGF@chitin film and bFGF@chitin films were implanted subcutaneously. Histological analysis showed that ChtBD-bFGF promoted vascularization at the implanted site more effectively than bFGF. These results suggest that the ChtBD-bFGF@chitin film is a stabile delivery vehicle for accelerating wound healing.
Copyright © 2017. Published by Elsevier Ltd.
 
Fig. 1
Fig. 1. Schematic illustration of ChtBD-bFGF binding to a chitinfilm for the wound healing. By applying a ChtBD-bFGF@chitin film on the skin wound site, the ChtBD-bFGF@chitin film acts as a wound dressing to promote cellularization and vascularization at the implanted site.
 

KEYWORDS:
Basic fibroblast growth factor; Chitin; Chitin binding domain; Vascularization; Wound dressing

PMID: 28821125 DOI: 10.1016/j.carbpol.2017.05.087
 

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