Adv. Funct. Mater. 2020, 2009258

伤口愈合延迟对患者、医疗保健和社会都有深远的影响。生长因子(GFs)是一种多功能的信号分子，可以协调伤口愈合过程中的一系列关键事件。由于没有单一的GF是伤口愈合效率的唯一决定因素，结合多剂量商用重组GFs的策略已被用于达到有效的治疗效果。然而，高昂的成本为广泛的临床应用设置了障碍。富血小板血浆(PRP)作为一种血源性产品，是从全血中提取的浓缩血小板，是再生医学中的一种创新工具，并已应用于临床。PRP凝胶的主要挑战仍然是在富含蛋白酶的创伤环境中给药后GFS的易感性分解和快速清除，其较弱的力学性能和随之而来的爆破释放效应限制了其应用和效果。

近日，北京化工大学的徐福建教授、李杨副教授以及中国人民解放军总医院第四医学中心的郝岱峰教授报道了一种通过简单的“一步法”激活过程构建基于PRP的双网络水凝胶（DN gel）。其良好的凝胶性和生长因子的缓释作用可能有利于临床应用。在大鼠身上的评估表明，DN凝胶通过快速上皮化、上调生长因子水平以及伤口愈合和血管生成阶段的早期过渡来促进伤口愈合。作为概念的证明，DN凝胶在猪伤口模型中也显示出优越的愈合效率。这些结果显示了这种水凝胶转化为下一代基于PRP的生物活性创面敷料的巨大潜力。

Delayed wound healing has a profound impact on patients, health care and society. Growth factors (GFS) are multifunctional signaling molecules that coordinate a series of key events in wound healing. Since no single GF is the only determinant of wound healing efficiency, the strategy of combining multiple doses of commercial recombinant GFS has been used to achieve effective therapeutic effect. However, the high cost has set up a barrier for a wide range of clinical applications. Platelet rich plasma (PRP), as a blood derived product, is a concentrated platelet extracted from whole blood. It is an innovative tool in regenerative medicine and has been applied in clinic. The main challenge of PRP gel is still the decomposition and rapid elimination of GFS after the administration of protease in the wound environment. Its weak mechanical properties and the consequent blasting release effect limit its application and effect.

Recently, Professor Xu Fujian of Beijing University of Chemical Technology, associate professor Li Yang and Professor Hao Daifeng of General Hospital of PLA fourth Medical Center reported a kind of PRP based dual network hydrogel (DN gel) by simple one step activation process. Its improved gelling property and sustained release of growth factors may be beneficial for clinical use. Evaluations in rats indicate that DN gel promote wound healing in terms of rapid re-epithelialization, up-regulated growth factor levels and early transitions in the wound healing and angiogenesis stages. As a proof of concept, DN gel also exhibits superior healing efficiency in a porcine wound model. These results demonstrate the great potential of transforming this hydrogel into the next generation of PRP-based bioactive wound dressing.

DOI:10.1002/adfm.202009258.

贠智赫