ACS Appl. Mater. Interfaces 2019, 11, 22730−22744

       急性创伤创口具有pH=6.4的酸性条件，哈佛医学院Li Yan教授团队使用微流体技术制备了掺杂碳酸钙粒子的海藻酸钠微球，并评估了流动相，表面活性剂，碳酸钙掺杂含量对微球的影响。随后使用台盼蓝作为药物模型，计算了不同碳酸钙含量的抗酸性与包封能力，通过对正常皮肤pH=7.4与急性创口pH=6.4进行对比，发现酸性条件下具有明显的缓释能力，推测酸性条件下碳酸钙降解产生的钙离子增强了水凝胶的交联程度。作者使用低碳酸钙掺杂量的抗生素微球与高碳酸钙掺杂量的bFGF微球等质量混合，作为测试微球。证明对金黄色葡萄球菌具有明显杀伤作用，且体外伤口愈合模型和动物模型证明具有生物相容性和促进皮肤修复的能力。

Acute trauma wounds have acidic conditions with pH=6.4. The author used microfluidic technology to prepare sodium alginate microspheres doped with calcium carbonate particles, and evaluated the effects of mobile phase, surfactant, and calcium carbonate doping content on the microspheres . Then, using trypan blue as a drug model, the acid resistance and encapsulation ability of different calcium carbonate contents were calculated. By comparing normal skin pH=7.4 with acute wound pH=6.4, it was found that it has obvious sustained-release ability under acidic conditions. It is speculated that the calcium ions produced by the degradation of calcium carbonate under acidic conditions enhance the degree of crosslinking of the hydrogel. The author used a mixture of antibiotic microspheres with low calcium carbonate doping content and bFGF microspheres with high calcium carbonate doping content as test microspheres. It is proved that it has obvious killing effect on Staphylococcus aureus, and the in vitro wound healing model and animal model prove that it has biocompatibility and the ability to promote skin repair.

DOI: 10.1021/acsami.9b04750

（李建超）