课题组发表文章 课题组发表文章

最小化 最大化

 

Angew Chem Int Ed Engl. 2016 Jul 6. doi: 10.1002/anie.201603155.

 A Bioorthogonal Approach for the Preparationof a Titanium-Binding Insulin-like Growth-Factor-1 Derivative by using Tyrosinase.

 Zhang C1,2,3, Miyatake H1, Wang Y3, Inaba T4, Wang Y2, Zhang P3, Ito Y5,6.

 Author information

 1 Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan.

 2 School of Pharmaceutical Sciences, Jilin University, No. 1266 Fujin Road, Changchun, Jilin, 130021, P.R. China.

 3 Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.

 4 Lipid Biology Laboratory, RIKEN, Japan.

5Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan. y-ito@riken.jp.

6Emergent Bioengineering Materials Research Team, RIKEN Center for Emergent Matter Science, 2-1 Hirosawa, Wako-shi, Saitama, 351-0198, Japan. y-ito@riken.jp.

 

Abstract

 The generation of metal surfaces with biological properties, such as cell-growth-enhancing and differentiation-inducing abilities, could be potentially exciting for the development of functional materials for use in humans, including artificial dental implants and joint replacements. However, currently the immobilization of proteins on the surfaces of the metals are limited. In this study, we have used a mussel-inspired bioorthogonal approach to design a 3,4-hydroxyphenalyalanine-containing recombinant insulin-like growth-factor-1 using a combination of recombinant DNA technology and tyrosinase treatment for the surface modification of titanium. The modified growth factor prepared in this study exhibited strong binding affinity to titanium, and significantly enhanced the growth of NIH3T3 cells on the surface of titanium.

KEYWORDS:

bioorthogonal chemistry; cell growth; growth factors; titanium; tyrosinase

 <Abstract>  <Full-htm> <Full-PDF