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多孔碳酸钙/羟基磷灰石微球体外及体内降解行为的研究
Colloids and Surfaces B: Biointerfaces 143 (2016) 56–63 全球大量骨病患者所接受的传统治疗，包括骨移植物及替代物的使用，具有诸多不足和缺点，如手术过程复杂冗长，慢性炎症，免疫反应等等，因此骨组织工程逐渐受到广泛的关注。制备出理想的适合组织细胞黏附、增殖及分化的组织工程支架材料目前仍然是一项严峻的挑战。羟基磷灰石（HA）被视为骨组织工程中的理想材料，主要是由于其化学组成与人体骨组织的无机组分相近似，且其具有优异的生物相容性、生物活性及骨传导性。然而HA的降解性较差，植入体内后会阻碍新生骨组织长入支架材料，因此其应用受到了一定的限制。 浙江科技大学的Zhong等研究人员采用了一种新颖的可注射、可降解HA基骨修复材料。他们采用丝胶蛋白调控制备碳酸钙（CaCO3）微球，并以其为模板，采用水热合成法制备CaCO3/HA复合微球，并研究了该复合微球体系的体外及体内降解行为。实验结果表明该复合微球的降解行为可以通过复合微球中CaCO3与HA比例的不同而调控，亦即通过水热反应时间调控，以匹配新生骨的长入速度，同时兼顾其生物相容性及生物活性，其有望成为骨组织工程中的一种优良的修复材料。 Bone tissue engineering has been paid much attentions because millions of patients undergo bone diseases globally while the traditional treatments of bone grafts and graft substitutes were faced with some disadvantages, including complicated surgical procedures, chronic inflammation, immune rejection, etc. The fabrication of ideal scaffold providing a suitable site for cell adhesion, proliferation and differentiation is still a major challenge. Hydroxyapatite (HA) is recognized as a preferable material for bone tissue engineering because its chemical composition is similar to the inorganic part of natural bone, and HA possessed excellent biocompatibility, bioactivity and osteoconductivity. But the poor degradability of HAP limits its application due to the impediment to prevent new bone from growing into the implant scaffolds. Zhong et al. from Zhejiang Sci-Tech University introduced a novel injectable and degradable hydroxyapatite-based bone repair material. CaCO3 regulated by silk sericin was utilized as a template to prepare porous CaCO3/hydroxyapatite composite microspheres throughhydrothermal treatment. In vitro and in vivo degradation behaviors of CaCO3 and hydroxyapatite microspheres were evaluated. The results indicated that the degradation pattern of the composite microspheres could be changed by adjusting the reaction time of hydrothermal treatment to match the growth speed of new bone and the composite materials showed an improved cytocompatibility, which might be a promising biomaterial for bone repair. （李林龙）
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