Biomaterials 287 (2022) 12166
人造肉具有使蛋白质生产方法多样化的潜力，但需要生产效率的创新才能使养殖肉成为可行的蛋白质替代品。微载体提供了在生物反应器中培养肉制品所需的足够体积的贴壁细胞的策略。然而，在不可食用的微载体上培养细胞需要额外的下游处理，以在消耗前解离细胞。在这里UCLA的 Amy C Rowat教授课题组展示了一种可食用的微载体，它们可以在单个生物反应器系统中支持肌原细胞的扩增和分化. 为了制造具有可扩展工艺的可食用微载体，使用油包水乳液作为明胶微粒的模板。还开发了一种新的压花技术，以压印具有凹槽拓扑的可食用微载体，以测试具有条纹表面纹理的微载体是否可以促进悬浮培养中的成肌细胞增殖和分化。
Cultured meat has potential to diversify methods for protein production, but innovations in production efficiency will be required to make cultured meat a feasible protein alternative. Microcarriers provide a strategy to culture sufficient volumes of adherent cells in a bioreactor that are required for meat products. However, cell culture on inedible microcarriers involves extra downstream processing to dissociate cells prior to consumption. Here, Professor Amy C Rowat who comes from UCLA present edible microcarriers that can support the expansion and differentiation of myogenic cells in a single bioreactor system. To fabricate edible microcarriers with a scalable process, we used water-in-oil emulsions as templates for gelatin microparticles. We also developed a novel embossing technique to imprint edible microcarriers with grooved topology in order to test if microcarriers with striated surface texture can promote myoblast proliferation and differentiation in suspension culture.
周小松