微球（microsphere）是指尺寸在数微米至数百微米的球状体，其成分清晰、结构简单、尺寸可控、比表面积大、便于后修饰，广泛应用于药物载体、组织再生修复和细胞三维培养等领域。在药物载体领域，来自苏州大学第一附属医院的陈亮等与上海交通大学医学院附属瑞金医院的崔文国教授团队合作在Nano Letters杂志上发表了题目为“Modulation of Local Overactive Inflammation via Injectable Hydrogel Microspheres ”的研究论文，作者通过在甲基丙烯酰化明胶（GelMA）上共价偶联APETx2多肽，进而负载髓核细胞，构建了“肽、细胞、水凝胶”复合微球，可调节局部炎性细胞因子风暴，又能调节细胞外基质（ECM）的代谢平衡，为过度活跃性炎症反应下的组织再生与修复提供了一种有效调控手段。
Microspheres ( microspheres ) are spherical bodies with sizes ranging from several microns to hundreds of microns. They have clear composition, simple structure, controllable size, large specific surface area and easy post-modification. They are widely used in drug carriers, tissue regeneration and cell three-dimensional culture. In the field of drug delivery, Chen Liang from the First Affiliated Hospital of Soochow University and Professor Cui Wenguo from Ruijin Hospital Affiliated to Shanghai Jiaotong University School of Medicine published a research paper entitled ‘ Modulation of Local Overactive Inflammation via Injectable Hydrogel Microspheres ‘ in Nano Letters. The author covalently coupled APETx2 peptide on methacryloyl gelatin ( GelMA ). The ‘ peptide, cell and hydrogel ‘ composite microspheres were constructed by loading nucleus pulposus cells, which can regulate the local inflammatory cytokine storm and the metabolic balance of extracellular matrix ( ECM ), and provide an effective means for tissue regeneration and repair under excessive active inflammatory response.
在组织修复领域，四川大学华西口腔医院的田卫东等利用静电滴注法担载了血小板裂解液(PL)和纳米粘土皂石于甲基丙烯酰化明胶(GelMA)微球中，提高了牙髓修复过程的新生血管形成。GelMA/PL/皂石复合微球可显著改善内部包封的人类牙髓干细胞（hDPSCs）的迁移、增殖和牙源性分化，体内微血管的形成和牙髓样组织的再生也得到促进。该论文Platelet lysate functionalized gelatin methacrylate microspheres for improving angiogenesis in endodontic regeneration发表在知名SCI杂志Acta Biomaterialia。
Although injectable hydrogel microsphere has demonstrated tremendous promise in clinical applications, local overactive in ﬂ ammation in degenerative diseases could jeopardize biomaterial implantation ’ s therapeutic e ﬃ cacy. Herein, an injectable“peptide-cell-hydrogel ” microsphere was constructed by covalently coupling of APETx2 and further loading of nucleus pulposus cells, which could inhibit local inﬂammatory cytokine storms to regulate the metabolic balance of ECM in vitro. The strategy provides an e ﬀ ective approach for tissue regeneration under overactive inﬂammatory responses.
In addition, human platelet lysate (PL), a natural-derived pool of multiple GFs, was incorporated into gelatin methacrylate (GelMA) microsphere system (GP), which was further modiﬁed by Laponite (GPL), a nanoclay with eﬃcient drug delivery ability. It could effectively induce tubule formation of human umbilical endothelial cells (HUVECs) and also promote human dental pulp stem cells (hDPSCs) migration. This work demonstrated that PL-incorporating GelMA microsphere system was a promising functional vehicle for promoting vascularized endodontic regeneration.