ACS Appl. Mater. Interfaces 2019, 11, 22730−22744
Acute trauma wounds have acidic conditions with pH=6.4. The author used microfluidic technology to prepare sodium alginate microspheres doped with calcium carbonate particles, and evaluated the effects of mobile phase, surfactant, and calcium carbonate doping content on the microspheres . Then, using trypan blue as a drug model, the acid resistance and encapsulation ability of different calcium carbonate contents were calculated. By comparing normal skin pH=7.4 with acute wound pH=6.4, it was found that it has obvious sustained-release ability under acidic conditions. It is speculated that the calcium ions produced by the degradation of calcium carbonate under acidic conditions enhance the degree of crosslinking of the hydrogel. The author used a mixture of antibiotic microspheres with low calcium carbonate doping content and bFGF microspheres with high calcium carbonate doping content as test microspheres. It is proved that it has obvious killing effect on Staphylococcus aureus, and the in vitro wound healing model and animal model prove that it has biocompatibility and the ability to promote skin repair.